Field Environmental Variation and Physiological Responses of Apostichopus japonicus to Major Winter Stressors: Implications for Overwintering Risk Management

Winter ice-cover periods pose substantial risks to Apostichopus japonicus aquaculture in northern China. We combined field monitoring of three representative aquaculture ponds between 2014 and 2017 with controlled single-factor laboratory exposures to low temperature, hypoxia, and hyposalinity in adult and juvenile sea cucumbers. Field observations revealed pronounced temporal, vertical, spatial, and interannual variability in water temperature, salinity, and dissolved oxygen (DO) during freezing and ice-melting periods. All three stressors caused clear deterioration in physiological condition, with juveniles generally exhibiting greater short-term sensitivity than adults under low-temperature and hypoxic exposure, whereas severe hyposalinity caused pronounced deterioration in both life stages. Lactate, malondialdehyde (MDA), and glutathione (GSH) showed distinct treatment- and time-dependent responses, consistent with stress-associated changes in anaerobic metabolism, lipid peroxidation, and glutathione-associated antioxidant status. Integration of field observations with laboratory responses indicated that water temperatures approaching 0 °C and salinities approaching approximately 20‰ represent environmentally relevant conditions associated with elevated overwintering risk. DO concentrations approaching approximately 3 mg/L may warrant intensified monitoring and management intervention, whereas 2 mg/L represents a more severe experimental hypoxia condition. The more extreme treatments of −2 °C and 15‰ should similarly be regarded as severe experimental scenarios rather than commonly occurring field conditions. These environmental values should therefore be interpreted as preliminary management-oriented risk references rather than definitive physiological thresholds. Overall, the study provides an empirical field-to-laboratory basis for biologically informed winter environmental monitoring and risk-based overwintering management of A. japonicus aquaculture.