Analytical validation of the Mindray BC-75R Vet hematology analyzer and comparison with the Siemens Advia 2120i for application in dogs and cats

The BC-75R Vet (Mindray Animal Medical) is a multi-species hematology analyzer that uses electrical impedance and SF-Cube technology (combining laser scatter, fluorescence, and 3-dimensional analysis) for CBC and reticulocyte analysis. We evaluated its analytical performance and compared it with the Advia 2120i (Siemens), a widely used analyzer in veterinary clinical pathology. CBCs were performed on EDTA-anticoagulated blood samples from 411 dogs and 225 cats submitted for routine testing. Analytical precision, linearity, carryover, and total observed error (TE obs ) were assessed according to ASVCP guidelines. We used a 2120i as the reference method and manual differential counts for WBC evaluation. RIs were calculated from 131 healthy dogs and 53 cats. The BC-75R Vet fulfilled the ASVCP requirements for repeatability (CVs < 5% for most variables) and linearity ( r > 0.97), with negligible carryover. TE obs values were within ASVCP allowable error limits, except for WBC types in QC material, which remained acceptable for in-clinic use. Agreement with the 2120i was acceptable for most RBC-related variables; however, systematic and proportional errors were found for mean corpuscular hemoglobin concentration (MCHC) in both species, and a systematic error for mean corpuscular volume (MCV) was found in dogs. Agreement for WBC and platelet counts was generally good, although discrepancies were noted for monocyte and mean platelet volume in both species. These differences likely reflect methodologic differences and known challenges in feline hematology. Despite these limitations, the BC-75R Vet met ASVCP standards and appears suitable for routine testing in dogs and cats. Manual smear review remains essential in selected cases.