Untargeted UHPLC‐Q‐Exactive Orbitrap MS and Targeted UHPLC‐QqQ‐MS/MS for Exploring the Effective Components of “Wen Tong Plaster” Cataplasm in Treating Rats With Primary Dysmenorrhea

{"\\"Wen":[0,39,119,153,181,256,275],"Tong":[1,40,120,154,182,257,276],"Plaster\\"":[2,41,121,155,183,258,277],"is":[3],"renowned":[4],"for":[5,255,286],"its":[6,19],"therapeutic":[7,253],"effects":[8],"of":[9,38,65,78,97,99,110,152,209,274,292],"warming":[10],"meridians,":[11],"dispelling":[12],"cold,":[13],"and":[14,22,43,49,61,74,136,140,162,168,190,200,216,269,283,294],"promoting":[15],"blood":[16],"circulation.":[17],"However,":[18],"chemical":[20,36,113],"composition":[21],"active":[23,80],"pharmacological":[24],"substances":[25],"have":[26],"yet":[27],"to":[28,93,170,242],"be":[29],"fully":[30],"elucidated.":[31],"In":[32,133,260],"this":[33],"study,":[34],"the":[35,44,62,66,75,95,118,196,206,212,235,243,262,265,290],"components":[37,46,69,82,114,126,145,229],"cataplasm":[42,122,156,184],"transdermal":[45,68,81,102,131,144],"in":[47,50,70,83,117,129,142,195,211,270],"vivo":[48,84,143],"vitro":[51,71,130,271],"were":[52,72,115,146,157,192,219],"comprehensively":[53],"analyzed":[54],"by":[55,159,203],"mass":[56],"spectrometry":[57],"targeted":[58],"quantification":[59],"method,":[60],"release":[63,150,178,272],"kinetics":[64,151,273],"main":[67],"evaluated,":[73],"concentration-time":[76,207],"variation":[77],"key":[79,100],"was":[85,91],"studied,":[86],"finally":[87],"molecular":[88,222],"docking":[89,223],"technology":[90],"used":[92],"predict":[94],"mechanism":[96,291],"action":[98,293],"bioactive":[101],"components.":[103],"Using":[104],"UHPLC-Q-Exactive":[105],"Orbitrap":[106],"MS,":[107],"a":[108,171,251,280],"total":[109],"129":[111],"plant":[112],"found":[116],"extract,":[123],"with":[124,234],"41":[125],"detected":[127],"as":[128,250],"compounds.":[132],"subcutaneous":[134,197,213],"tissue":[135,202,218],"uterine":[137],"tissue,":[138,198,214],"53":[139],"36":[141],"characterized,":[147],"respectively.":[148],"The":[149,221],"evaluated":[158],"tetrahydropalmatine,":[160,186],"corydaline,":[161,187],"dihydrotanshinone":[163,188],"I,":[164,189],"three":[165],"marker":[166],"components,":[167],"fitted":[169],"first-order":[172],"kinetic":[173],"model":[174],"based":[175],"on":[176,289],"integrated":[177],"kinetics.":[179],"After":[180],"administration,":[185],"α-cyperone":[191],"accurately":[193],"quantified":[194],"plasma,":[199,215],"uterus":[201,217],"UHPLC-QqQ-MS/MS,":[204],"while":[205],"changes":[208],"them":[210],"investigated.":[220],"results":[224],"indicated":[225],"that":[226],"all":[227],"four":[228],"exhibited":[230],"favorable":[231],"binding":[232,240],"affinity":[233],"targets,":[236],"particularly":[237],"demonstrating":[238],"strong":[239],"capacity":[241],"cytochrome":[244],"P450":[245],"1A1":[246],"target,":[247],"suggesting":[248],"it":[249],"potential":[252],"target":[254],"cataplasm.":[259],"summary,":[261],"study":[263],"revealed":[264],"pharmacodynamic":[266],"material":[267],"basis":[268],"cataplasm,":[278],"providing":[279],"theoretical":[281],"foundation":[282],"data":[284],"support":[285],"further":[287],"research":[288],"clinical":[295],"application.":[296]}

Authors

Institutions

Publication Details

Journal
Journal of Separation Science
Published
2026-09-01
DOI
https://doi.org/10.1002/jssc.70508
Primary Topic
Menstrual Health and Disorders
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
article

Untargeted UHPLC‐Q‐Exactive Orbitrap MS and Targeted UHPLC‐QqQ‐MS/MS for Exploring the Effective Components of “Wen Tong Plaster” Cataplasm in Treating Rats With Primary Dysmenorrhea

Beibei Yu, Longyun Duan, Xinjun Zhang, Wenjing Hou et al.
Journal of Separation Science
Menstrual Health and Disorders
article

Untargeted UHPLC‐Q‐Exactive Orbitrap MS and Targeted UHPLC‐QqQ‐MS/MS for Exploring the Effective Components of “Wen Tong Plaster” Cataplasm in Treating Rats With Primary Dysmenorrhea

Beibei Yu, Longyun Duan, Xinjun Zhang, Wenjing Hou, Jin Liu, Wenjing Chen, Zaiyun Sui, Ying Li, Zongtong Yang, Yufei Li, Tong Zhang
article en

Abstract

"Wen Tong Plaster" is renowned for its therapeutic effects of warming meridians, dispelling cold, and promoting blood circulation. However, its chemical composition and active pharmacological substances have yet to be fully elucidated. In this study, the chemical components of "Wen Tong Plaster" cataplasm and the transdermal components in vivo and in vitro were comprehensively analyzed by mass spectrometry targeted quantification method, and the release kinetics of the main transdermal components in vitro were evaluated, and the concentration-time variation of key active transdermal components in vivo was studied, finally molecular docking technology was used to predict the mechanism of action of key bioactive transdermal components. Using UHPLC-Q-Exactive Orbitrap MS, a total of 129 plant chemical components were found in the "Wen Tong Plaster" cataplasm extract, with 41 components detected as in vitro transdermal compounds. In subcutaneous tissue and uterine tissue, 53 and 36 in vivo transdermal components were characterized, respectively. The release kinetics of "Wen Tong Plaster" cataplasm were evaluated by tetrahydropalmatine, corydaline, and dihydrotanshinone I, three marker components, and fitted to a first-order kinetic model based on integrated release kinetics. After "Wen Tong Plaster" cataplasm administration, tetrahydropalmatine, corydaline, dihydrotanshinone I, and α-cyperone were accurately quantified in the subcutaneous tissue, plasma, and uterus tissue by UHPLC-QqQ-MS/MS, while the concentration-time changes of them in the subcutaneous tissue, plasma, and uterus tissue were investigated. The molecular docking results indicated that all four components exhibited favorable binding affinity with the targets, particularly demonstrating strong binding capacity to the cytochrome P450 1A1 target, suggesting it as a potential therapeutic target for "Wen Tong Plaster" cataplasm. In summary, the study revealed the pharmacodynamic material basis and in vitro release kinetics of "Wen Tong Plaster" cataplasm, providing a theoretical foundation and data support for further research on the mechanism of action and clinical application.

Journal of Separation ScienceVol. 49(9)
Shandong University of Traditional Chinese Medicine (CN), State Administration of Traditional Chinese Medicine of the People's Republic of China (CN), Shandong Academy of Chinese Medicine (CN)
Openalex Percentile: Top 9%
Menstrual Health and Disorders
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.