Impact of interrupted and continuous suturing techniques on perfusion in rat JV-CCA End-to-Side anastomosis

Abstract Purpose End-to-side (ETS) anastomosis is a prevalent approach in cerebral revascularization; nevertheless, the effects of interrupted suturing (IS) versus continuous suturing (CS) methods on perfusion in tiny vessels are still contentious. This study aims to examine the effects of IS and CS procedures on the perfusion of the jugular vein (JV) to common carotid artery (CCA) ETS anastomosis in rats. Methods Sixteen rats were randomly allocated into two groups to undergo ETS anastomosis via IS or CS. The laser speckle contrast imaging equipment was employed for postoperative perfusion evaluation by delineating the region of interest at the proximal and distal ends of the anastomosed artery and JV immediately postoperation, as well as at 1, 2, 4, and 6 h thereafter. Results No statistically significant difference was observed in the diameter of the anastomotic stoma between the two groups. The perfusion volume of the donor and recipient vasculature increased throughout time, especially during the first two postoperative hours. No statistically significant differences were observed in vascular perfusion at both the proximal and distal ends of the anastomosed artery, as well as at JV, between the IS and CS groups at multiple monitoring intervals during a 6-h postoperative timeframe. Conclusions The current short-term study indicates that there are no statistically significant differences in the perfusion of donor and recipient arteries shortly after surgery between the IS and CS groups in the JV-CCA ETS model in rats.