Development and validation of the 6-dye lyophilized fluorescent multiplex PCR system
{"Aiming":[0],"to":[1,106],"address":[2],"the":[3,78,111,144,161],"issues":[4],"associated":[5],"with":[6,130],"traditional":[7,124],"liquid":[8,125,132],"STR":[9,40],"reagents-such":[10],"as":[11],"dependence":[12],"on":[13,61,163],"cold-chain":[14,164],"storage,":[15],"cumbersome":[16],"operation,":[17],"and":[18,42,55,102,152,170,182],"unsuitability":[19],"for":[20,92,118,146,173],"on-site":[21,175],"rapid":[22,174],"detection-this":[23],"study":[24],"has":[25,71],"successfully":[26],"developed":[27],"a":[28,57,153],"six-color":[29],"fluorescent":[30],"lyophilized":[31,79],"multiplex":[32],"amplification":[33],"reagent.":[34],"The":[35],"reagent":[36,69,80,158,171],"includes":[37],"29":[38],"autosomal":[39],"loci":[41],"1":[43],"gender":[44],"identification":[45],"locus.":[46],"By":[47],"screening":[48],"high-tolerance":[49],"Taq":[50],"polymerase,":[51],"optimizing":[52],"lyoprotectant":[53],"formulations,":[54],"developing":[56],"lyophilization":[58],"process":[59],"based":[60],"thermodynamic":[62],"analysis,":[63],"stable":[64,91],"ambient-temperature":[65],"storage":[66],"of":[67,123],"key":[68],"components":[70],"been":[72],"achieved.":[73],"Performance":[74],"validation":[75],"demonstrates":[76],"that":[77,122],"exhibits":[81],"high":[82],"sensitivity":[83],"(100%":[84],"detection":[85,116,139],"at":[86,95],"0.125":[87],"ng":[88],"DNA),":[89],"remains":[90],"60":[93],"days":[94],"room":[96],"temperature,":[97],"rehydrates":[98],"rapidly":[99],"(<1":[100],"second),":[101],"shows":[103],"some":[104],"tolerance":[105],"common":[107],"inhibitors.":[108],"Benefiting":[109],"from":[110],"\\"whole-volume":[112],"loading\\"":[113],"advantage,":[114],"its":[115],"capability":[117],"trace":[119],"samples":[120],"surpasses":[121],"reagents.":[126],"In":[127],"parallel":[128],"comparison":[129],"similar":[131],"reagents,":[133],"it":[134],"achieves":[135],"completely":[136],"consistent":[137],"locus":[138],"rates":[140],"(100%),":[141],"fully":[142],"meeting":[143],"requirements":[145],"individual":[147],"identification.":[148],"With":[149],"simple":[150],"operation":[151],"ready-to-use,":[154],"disposable":[155],"design,":[156],"this":[157],"effectively":[159],"overcomes":[160],"reliance":[162],"logistics.":[165],"It":[166],"provides":[167],"robust":[168],"technical":[169],"support":[172],"forensic":[176],"testing,":[177],"emergency":[178],"response,":[179],"grassroots":[180],"applications,":[181],"DNA":[183],"database":[184],"construction,":[185],"demonstrating":[186],"significant":[187],"application":[188],"prospects.":[189]}
Authors
- Lina Jia
- Cui Yuling
- Ming Ji
- Jing Wang
- Zhe Zhang
- Wen Song
- Hao Yu
- Lei Shang
- Xue Bai
Institutions
- Hebei Medical University (CN)
- Ministry of Public Security of the People's Republic of China (CN)
- Institute of Forensic Science (CN)
Publication Details
- Journal
- PLoS ONE
- Published
- 2026-08-27
- DOI
- https://doi.org/10.1371/journal.pone.0356258
- Primary Topic
- Forensic and Genetic Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Special Funds for the Basic Research and Development Program in the Central Non-profit Research Institutesof China
- Institute of Forensic Science, Ministry of Public Security