Protocatechualdehyde from Taraxacum officinale Acted as A Competitive Substrate to Inhibit Xanthine Oxidase Attenuates Hyperuricemia
{"Abstract":[0],"The":[1],"escalating":[2],"prevalence":[3],"of":[4,42,48,198],"hyperuricemia":[5],"(HUA)":[6],"poses":[7],"a":[8,60,82,97,104,108,117,135,158,239],"formidable":[9],"challenge":[10],"to":[11,163,252],"global":[12],"public":[13,256],"health.":[14],"As":[15,107],"the":[16,29,36,46,154,168,194,218,254],"key":[17,159],"rate-limiting":[18],"enzyme":[19],"in":[20,113,190,212],"uric":[21],"acid":[22,133],"(UA)":[23],"biosynthesis,":[24],"xanthine":[25,124],"oxidase":[26],"(XOD)":[27],"remains":[28],"primary":[30],"target":[31],"for":[32,116,161,247],"therapeutic":[33],"intervention.":[34],"However,":[35],"inherent":[37],"toxicity":[38],"and":[39,54,120,137,181,208,223,241],"adverse":[40],"effects":[41],"existing":[43],"pharmacotherapies":[44],"necessitate":[45],"exploration":[47],"safe,":[49],"natural":[50,75],"alternatives.":[51],"We":[52],"identified":[53],"isolated":[55],"protocatechuic":[56,132],"aldehyde":[57,155,169],"(PCA)":[58],"as":[59,81,177,238],"highly":[61],"potent":[62],"XOD":[63,110,179,183,206],"inhibitor":[64],"(IC₅₀=0.250":[65],"mM)":[66],"from":[67,144],"Taraxacum":[68],"officinale":[69],"(TO).":[70],"It":[71],"significantly":[72,204],"outperforming":[73],"established":[74],"flavonoids.":[76],"Mechanistically,":[77],"PCA":[78,114,127,162,173,202,216],"uniquely":[79],"functions":[80],"\\"slow-turnover":[83],"substrate\\"":[84],"via":[85],"its":[86,175,182],"C1-aldehyde":[87],"group.":[88],"This":[89],"high-affinity":[90],"binding":[91],"coupled":[92],"(Km":[93],"=14":[94],"μM)":[95],"with":[96],"slow":[98],"catalytic":[99],"turnover":[100],"(Kcat=0.014":[101],"s−1)":[102],"created":[103],"\\"kinetic":[105],"trap\\".":[106],"result,":[109],"tied":[111],"up":[112],"processing":[115],"long":[118],"period":[119],"thus":[121],"continuously":[122],"blocking":[123],"oxidation.":[125],"Furthermore,":[126,153],"was":[128,157,171],"enzymatically":[129],"oxidized":[130],"into":[131],"that":[134,149,201],"safe":[136,240],"bioactive":[138],"metabolite,":[139],"which":[140],"strictly":[141],"distinguishes":[142],"it":[143],"clinical":[145],"drugs":[146],"like":[147],"allopurinol":[148],"generate":[150],"toxic":[151],"intermediates.":[152],"group":[156,160,170],"exert":[164],"this":[165],"mechanism.":[166],"When":[167],"substituted,":[172],"loses":[174],"function":[176],"an":[178],"substrate,":[180],"inhibition":[184],"activity":[185,207],"decreases":[186],"by":[187],"12.35-fold.":[188],"Subsequent":[189],"vivo":[191],"studies":[192],"validated":[193],"attenuates":[195],"HUA":[196,213],"efficacy":[197],"PCA,":[199],"demonstrating":[200],"intervention":[203,246],"suppressed":[205],"reduced":[209],"UA":[210],"level":[211],"mice.":[214],"Consequently,":[215],"restored":[217],"disrupted":[219],"oxidative":[220],"stress-inflammatory":[221],"homeostasis":[222],"ameliorated":[224],"HUA-induced":[225],"renal":[226],"pathological":[227],"damage.":[228],"Overall,":[229],"our":[230],"work":[231],"provided":[232],"strong":[233],"scientific":[234],"evidence":[235],"supporting":[236],"TO":[237],"effective":[242],"dietary":[243],"supplement":[244],"or":[245],"managing":[248],"HUA,":[249],"thereby":[250],"helping":[251],"address":[253],"related":[255],"health":[257],"challenges.":[258]}
Authors
- Mingming Li (ORCID: https://orcid.org/0000-0001-8887-9299)
- Mintong Tian
- Yongzhi Han
- Kang Xu
- Jia Wang
- Jun Wu
- Ce Liu
- Qipeng Yuan
- Xinxiao Sun
- Yuan Rao
- Xin Yu
- Yulong Li
Institutions
- Beijing University of Chemical Technology (CN)
Publication Details
- Journal
- Food Science and Human Wellness
- Published
- 2026-08-26
- DOI
- https://doi.org/10.26599/fshw.2026.9251236
- Primary Topic
- Gout, Hyperuricemia, Uric Acid
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Beijing University of Chemical Technology
- Beijing Nova Program
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities