The Escherichia coli uhp two-component system functions as a sensory channel for hexose phosphates secreted by Bifidobacterium

{"The":[0],"human":[1,115,181],"gut":[2],"microbiota":[3],"relies":[4],"on":[5,113,175],"inter-species":[6],"communication":[7],"for":[8],"stability,":[9],"yet":[10],"interactions":[11],"between":[12],"non-dominant":[13],"taxa":[14],"like":[15],"Pseudomonadota":[16],"(Escherichia":[17],"coli)":[18],"and":[19,52,153,170],"Actinomycetota":[20],"(Bifidobacterium)":[21],"remain":[22],"poorly":[23],"understood.":[24],"We":[25,138],"demonstrate":[26],"that":[27,50,85,100],"E.":[28],"coli":[29],"repurposes":[30],"its":[31],"Uhp":[32,168],"two-component":[33],"system":[34,169],"as":[35],"a":[36,61,76,104,134,144,172],"specialized":[37],"sensory":[38],"channel":[39],"to":[40,150],"monitor":[41],"Bifidobacterium":[42,59],"metabolic":[43,136],"activity.":[44],"Screening":[45],"54":[46],"transport":[47],"systems":[48],"revealed":[49],"uhpT":[51],"idnT":[53,123],"genes":[54],"are":[55],"specifically":[56],"induced":[57],"by":[58],"supernatants,":[60],"response":[62],"absent":[63],"in":[64],"members":[65],"of":[66,107,127,133,166],"the":[67,89,131,157,163,167,176,180],"Bacteroidota.":[68],"Using":[69],"an":[70],"enzymatic":[71],"\\"kinetic":[72],"trap\\"":[73],"approach":[74],"with":[75],"slow-acting":[77],"Zwf":[78],"mutant":[79],"(D177N),":[80],"we":[81],"provide":[82],"biochemical":[83],"evidence":[84],"glucose-6-phosphate":[86],"(G6P)":[87],"is":[88,103],"primary":[90],"signaling":[91],"molecule.":[92],"Experimental":[93],"validation":[94],"using":[95],"Modified":[96],"Garche's":[97],"Medium":[98],"confirms":[99],"G6P":[101],"secretion":[102,132],"consistent":[105],"byproduct":[106],"bifidobacterial":[108],"metabolism":[109,152],"occurring":[110],"during":[111],"growth":[112],"diverse":[114],"milk":[116],"oligosaccharide":[117],"(HMO)":[118],"constituents,":[119],"including":[120],"galactose.":[121],"Furthermore,":[122],"activation":[124],"occurs":[125],"independently":[126],"Uhp-mediated":[128],"transport,":[129],"suggesting":[130],"multifaceted":[135],"cocktail.":[137],"propose":[139],"these":[140],"molecular":[141],"links":[142],"form":[143],"\\"non-dominant":[145],"network\\"":[146],"enabling":[147],"low-abundance":[148],"species":[149],"coordinate":[151],"maintain":[154],"stability":[155],"alongside":[156],"dominant":[158],"majority.":[159],"These":[160],"findings":[161],"redefine":[162],"physiological":[164],"role":[165],"offer":[171],"new":[173],"perspective":[174],"regulatory":[177],"networks":[178],"governing":[179],"microbiome.":[182],"(183":[183],"words).":[184]}

Authors

Institutions

Publication Details

Journal
Scientific Reports
Published
2026-08-25
DOI
https://doi.org/10.1038/s41598-026-64276-3
Primary Topic
Bacterial Genetics and Biotechnology
Type
article
Field-Weighted Citation Impact
0.00

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
article

The Escherichia coli uhp two-component system functions as a sensory channel for hexose phosphates secreted by Bifidobacterium

Kaneyoshi Yamamoto, Miho Yoshimura, Masanori Tamura, Masato Hoshino et al.
Scientific Reports
Bacterial Genetics and Biotechnology
article

The Escherichia coli uhp two-component system functions as a sensory channel for hexose phosphates secreted by Bifidobacterium

Kaneyoshi Yamamoto, Miho Yoshimura, Masanori Tamura, Masato Hoshino, Tomotsugu Ohno, Tsubasa Niino
article en

Abstract

The human gut microbiota relies on inter-species communication for stability, yet interactions between non-dominant taxa like Pseudomonadota (Escherichia coli) and Actinomycetota (Bifidobacterium) remain poorly understood. We demonstrate that E. coli repurposes its Uhp two-component system as a specialized sensory channel to monitor Bifidobacterium metabolic activity. Screening 54 transport systems revealed that uhpT and idnT genes are specifically induced by Bifidobacterium supernatants, a response absent in members of the Bacteroidota. Using an enzymatic "kinetic trap" approach with a slow-acting Zwf mutant (D177N), we provide biochemical evidence that glucose-6-phosphate (G6P) is the primary signaling molecule. Experimental validation using Modified Garche's Medium confirms that G6P secretion is a consistent byproduct of bifidobacterial metabolism occurring during growth on diverse human milk oligosaccharide (HMO) constituents, including galactose. Furthermore, idnT activation occurs independently of Uhp-mediated transport, suggesting the secretion of a multifaceted metabolic cocktail. We propose these molecular links form a "non-dominant network" enabling low-abundance species to coordinate metabolism and maintain stability alongside the dominant majority. These findings redefine the physiological role of the Uhp system and offer a new perspective on the regulatory networks governing the human microbiome. (183 words).

Scientific ReportsVol. 16(1)
Hosei University (JP)
Ministry of Education, Culture, Sports, Science and Technology
Life in Land
Openalex Percentile: Top 11%
Bacterial Genetics and Biotechnology
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.