Fabrication and Characterization of Electrospun Polyacrylonitrile/Polyaniline–Graphene Oxide Nanoscroll Nanofiber Composite for Potential Glucose Sensing Applications

Wearable sweat biosensors require electrode materials with high surface area, conductivity and mechanical compliance, yet chemically polymerized polyaniline forms dense, low-surface-area films with limited flexibility. In this work, flexible free-standing nanofiber mats were fabricated by coaxial electrospinning, using a sulfuric-acid-doped polyacrylonitrile/polyaniline (PAN/PANI) core and a shell containing graphene oxide nanoscrolls (GONS) at 1 and 3 wt%, followed by gold nanoparticle and ferrocene incorporation, glucose oxidase (GOx) immobilization and a Nafion coating. Scanning electron microscopy showed uniform bead-free fibers with an interconnected pore network and an apparent image-derived porosity of approximately 40%. Energy-dispersive X-ray spectroscopy confirmed the uniform distribution of carbon, oxygen, nitrogen and sulfur across the matrix. Fourier-transform infrared spectroscopy retained the nitrile band at 2243 cm−1 and the quinoid and benzenoid bands at 1547 and 1476 cm−1, while amide bands at 1730 and 1641 cm−1 confirmed retention of protein from enzymes. X-ray diffraction gave crystallinities of 76.6% for GONS and 60% for the pure PANI. Four-point probe measurements showed conductivity increasing from 0.0481 S/cm to 1 wt% GONS to 0.0595 S/cm for the 3 wt% mat with additives. These material and structural characterizations establish a promising foundation for future electrochemical validation and sensor development.