HPV genotype distribution and associated risk factors in women from Karad, Maharashtra: A molecular characterisation study

Human papillomavirus (HPV) genotype distribution varies regionally, influencing vaccination and screening strategies. This molecular characterisation study determined HPV prevalence and circulating genotypes among 225 women (≥30 y) attending gynaecological clinics in Karad, Satara district, Maharashtra. HPV DNA was detected in 14 participants (6.2%) using polymerase chain reaction (PCR) and sequencing. Identified genotypes included HPV-6, HPV-59, HPV-66, and HPV-81, indicating circulation of both low-risk and high-risk types. Multiple sexual partners showed the strongest association with HPV infection. These preliminary findings highlight the need for region-specific genotype surveillance to inform prevention strategies.