Mycoplasma mycoides subsp. capri encodes multiple pathways for hydrogen peroxide production
Summary Several Mollicutes species import glycerol and related molecules as carbon sources, resulting in the release of hydrogen peroxide (H 2 O 2 ), which has been suggested as a pathogenicity mechanism. We systematically deleted genes encoding components of the H 2 O 2 pathways in Mycoplasma mycoides subsp. capri GM12 to get insight into the role of candidate factors constituting the pathways. As expected, glycerol metabolism in GM12 is associated with the highest H 2 O 2 production compared with glycerophosphocholine. The absence of either L-alpha-glycerophosphate oxidase (GlpO) or the glycerol uptake facilitator GlpF, which is the main glycerol importer in GM12, ceased H 2 O 2 production. However, our data did not support any role for the presumed glycerol transport system, GtsABC in glycerol- or glycerophosphocholine-dependent H 2 O 2 production. Caprine peripheral blood mononuclear cells (PBMCs) did not show increased cytotoxic effects upon incubation with GM12 in the presence of glycerol, which does not support H 2 O 2 to be a primary pathogenicity mechanism in GM12.
Authors
- Sanjay Vashee (ORCID: https://orcid.org/0000-0001-6374-438X)
- Pascal Sirand‐Pugnet (ORCID: https://orcid.org/0000-0003-2613-0762)
- Laurent Falquet (ORCID: https://orcid.org/0000-0001-8102-7579)
- Norberto González-Juarbe (ORCID: https://orcid.org/0000-0003-2481-698X)
- Nacyra Assad-Garcia (ORCID: https://orcid.org/0000-0001-5270-2272)
- Alain Blanchard (ORCID: https://orcid.org/0000-0003-4223-5185)
- Thatcha Yimthin (ORCID: https://orcid.org/0009-0004-4489-172X)
- Joerg Jores (ORCID: https://orcid.org/0000-0003-3790-5746)
- Carole Lartigue (ORCID: https://orcid.org/0000-0001-5550-7579)
- Suchismita Chandran
- Thomas Démoulins (ORCID: https://orcid.org/0000-0003-0654-5813)
- Jing Zhang (ORCID: https://orcid.org/0000-0002-5442-7793)
- Ray-Yuan Chuang
- Nina Alperovich
Institutions
- University of Bern (CH)
- SIB Swiss Institute of Bioinformatics (CH)
- J. Craig Venter Institute (US)
- University of Fribourg (CH)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Biologie du Fruit et Pathologie (FR)
Publication Details
- Journal
- iScience
- Published
- 2026-08-20
- DOI
- https://doi.org/10.1016/j.isci.2026.117211
- Primary Topic
- Microbial infections and disease research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung