Development of low‐prolamin rice germplasm via CRISPR/Cas9 editing to improve eating and cooking quality
Simultaneous editing of two major prolamin-encoding genes in rice using a single-guide RNA suppressed prolamin accumulation and reconfigured seed storage protein composition. This targeted modification markedly improved rice eating and cooking quality while maintaining the levels of total starch, total protein, and key agronomic traits.
Authors
- Gexing Wan (ORCID: https://orcid.org/0000-0002-1993-495X)
- Zhichao Zhao (ORCID: https://orcid.org/0000-0002-0994-6922)
- Zhihui Chen (ORCID: https://orcid.org/0000-0002-1380-4804)
- Bingke Luo
- Yulong Ren (ORCID: https://orcid.org/0000-0002-9032-3529)
- Hui Dong (ORCID: https://orcid.org/0009-0006-4559-1440)
- Tian Pan (ORCID: https://orcid.org/0000-0001-6107-4175)
- Yiyang Zhou (ORCID: https://orcid.org/0000-0001-8956-3229)
- Cailin Lei (ORCID: https://orcid.org/0000-0002-8473-1948)
- Jianmin Wan (ORCID: https://orcid.org/0000-0002-7813-4362)
- Yu Chen (ORCID: https://orcid.org/0000-0003-1305-7594)
- Jie Wang (ORCID: https://orcid.org/0000-0002-3979-1810)
- Xin Wang (ORCID: https://orcid.org/0000-0003-1142-3562)
- Wenkun Yang
- Xiaohang Han
- Yun Zhu (ORCID: https://orcid.org/0000-0003-3675-8430)
- Lianji Lu
Institutions
- Nanjing Agricultural University (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Institute of Crop Sciences (CN)
- Henan Agricultural University (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2026-08-20
- DOI
- https://doi.org/10.1111/jipb.70377
- Primary Topic
- Transgenic Plants and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Chinese Academy of Agricultural Sciences
- Earmarked Fund for China Agriculture Research System