Autofluorescence profiles define depot-specific adipocyte phenotypes in perivascular and non-perivascular adipose tissue

= 6), we assessed intrinsic fluorescence in adipocytes from PVATs of thoracic and abdominal aorta, and from mesenteric arteries alongside phenotype-matched non-PVAT counterparts: interscapular brown, subcutaneous, and retroperitoneal tissues. Using optimized methods to maintain adipocyte integrity, we demonstrate that thoracic and intrascapular exhibit similar autofluorescence profiles. In contrast, PVAT sites mesenteric and abdominal diverge across the yellow, red, and violet channels compared with non-PVAT depots, retroperitoneal and subcutaneous. In conclusion, PVAT and non-PVAT adipocytes exhibit depot-specific spectral patterns, with higher autofluorescence observed in PVAT cells, validating autofluorescence as a marker-independent approach for identifying adipocyte differences.