Key challenges for <i>in vitro</i> testing of oral tobacco and nicotine products for regulatory applications: Recommendations for using <i>in vitro</i> toxicity and disease models
The Institute for In Vitro Sciences (IIVS) has sponsored a series of workshops with the aim of developing recommendations for scientific and technical approaches to conducting in vitro assays to assess potential toxicity within and across tobacco and nicotine products. As well as providing a background overview of oral tobacco and nicotine products, this report focuses on the methods used to generate extracts from these products for in vitro testing, the recommended best testing practices, and suggested areas for future research. This publication was developed by a working group of workshop members, in conjunction with the ninth IIVS in vitro workshop entitled ‘ In Vitro Models for Testing Oral Tobacco and Nicotine Products and Continued Discussion of In Vitro Models of Toxicity and Disease ’, which was held in January 2024.
Authors
- Jacqueline Miller‐Holt (ORCID: https://orcid.org/0000-0001-5815-0552)
- Jeremie Gafner
- Rana Tayyarah (ORCID: https://orcid.org/0000-0001-8339-0275)
- Emma Bishop (ORCID: https://orcid.org/0000-0002-9048-1527)
- Katarina Aleksa (ORCID: https://orcid.org/0009-0007-8756-1562)
- Roman Wieczorek (ORCID: https://orcid.org/0000-0003-2826-1539)
- Sara Moses
- Hans Raabe
- Mitchell Klausner (ORCID: https://orcid.org/0000-0003-0651-6895)
- Sara Hurtado
- M. Hollings
- Martha M. Moore
- Rodger Curren (ORCID: https://orcid.org/0000-0002-6947-2589)
- Kristie Sullivan
- Samuel Constant
- Leon F. Stankowski
- Robert Leverette
- Lynn McFadden
- Damien Breheny
- Brian M. Keyser
- Mariano Scian
Institutions
- Exelixis (United States) (US)
- Centre for Innovation in Regulatory Science (GB)
- Institute for In Vitro Sciences (US)
- Martel (CH)
- MatTek Corporation (United States) (US)
- Charles River Laboratories (United States) (US)
- Research Applications (United States) (US)
- Harrogate District Hospital (GB)
- British American Tobacco (Germany) (DE)
- Orthopaedic Research of Virginia (US)
- Imperial Brands (United Kingdom) (GB)
- Altria (United States) (US)
- Nutrition International (CA)
- Ashland (United States) (US)
Publication Details
- Journal
- Alternatives to Laboratory Animals
- Published
- 2026-08-14
- DOI
- https://doi.org/10.1177/02611929261475613
- Primary Topic
- Smoking Behavior and Cessation
- Type
- article
- Field-Weighted Citation Impact
- 0.00