Softwood nanocellulose–silver nanoparticle membranes for burn wound healing in rabbits

In this study, the efficacy of pinus sp. nanocellulose membranes, with and without silver nanoparticles, was evaluated in the treatment of experimental burns in rabbits. A total of twenty animals were used, each receiving three standardized dorsal burns of equal size, treated with either a simple dressing (negative control), a nanocellulose membrane without silver, or a nanocellulose membrane containing silver. Healing was monitored through macroscopic and microscopic analyses performed on days 3, 7, 14, and 30 after the induction of the burns. The findings demonstrated that nanocellulose membranes effectively promoted tissue repair and skin regeneration, with evidence of fibroblast activity, collagen deposition, and neovascularization throughout the healing process. The incorporation of silver nanoparticles showed potential to enhance early wound contraction, although all groups achieved complete re-epithelialization by the end of the experimental period. While the addition of silver may contribute to antimicrobial protection and early tissue response, further studies are required to confirm its role in accelerating the healing process and to better elucidate its mechanisms of action. Overall, pinus sp. nanocellulose membranes represent a safe and effective alternative for the treatment of burns, supporting their potential application in clinical practice.