Intraoral Radiographic Description of Dental Anatomy and Pathology in the Virginia Opossum ( <i>Didelphis virginiana</i> )

) has been described, the radiographic appearance of the dentition and the prevalence of dental and oral pathology have not been comprehensively evaluated. The objective of this study was to characterize the intraoral radiographic dental anatomy of the Virginia opossum and to determine the prevalence and distribution of anatomic variations and pathologic conditions affecting the dentition. Oral examination, standardized photography, and full-mouth intraoral dental radiography were performed on 25 deceased free-ranging opossums obtained from a wildlife rehabilitation hospital. Findings were systematically recorded, including the presence of developmental variations, malocclusion, periodontitis, endodontic disease, and tooth resorption. Dental anatomical or developmental variations were identified in 76% of specimens, with missing teeth being the most frequent finding. Supernumerary teeth were uncommon at 8% of specimens and restricted to the mandibular first premolars. Root morphology variations were frequently observed, including convergent premolar roots in 44% of adult specimens and mandibular root dilaceration in 20%. Class III malocclusion with dental attrition occurred in 16% of specimens. Endodontic disease affected 80% of opossums. Apical obliteration of the canine pulp canal was identified in 76% of adult canine teeth and likely represents a species or age-related variation. Dental fractures were common, particularly in canine teeth, at 80%. Periodontitis was identified in 24% of opossums, primarily affecting molars. These findings establish baseline radiographic anatomy and disease prevalence to support diagnosis and treatment planning in wildlife and veterinary care.