Isolation and optimization of L-Asparaginase production from native halotolerant <i>Bacillus subtilis</i> bacteria isolated from Central Iran
strains are capable of producing L-Asparaginase. PEG precipitation is a suitable method for initial purification, and statistical optimization successfully identified the critical culture conditions to enhance enzyme yield significantly. These strains represent promising sources for the production of this clinically valuable enzyme.
Authors
- Parvin Akbarzadehlaleh (ORCID: https://orcid.org/0000-0002-6587-007X)
- Azita Dilmaghani (ORCID: https://orcid.org/0000-0001-6556-8332)
- Kamran Hosseini (ORCID: https://orcid.org/0000-0003-1189-7143)
- Somayeh Hallaj‐Nezhadi (ORCID: https://orcid.org/0000-0003-4730-205X)
- Mojtaba Khyrallahpour (ORCID: https://orcid.org/0009-0003-7014-5053)
- Mohsen Hassanpour (ORCID: https://orcid.org/0009-0007-9990-7727)
Institutions
- Tabriz University of Medical Sciences (IR)
- Shiraz University of Medical Sciences (IR)
- Cardiovascular Research Center (BR)
- Tropical Diseases Research Centre (ZM)
- Hospital for Tropical Diseases (VN)
- Pharmaceutical Biotechnology (Czechia) (CZ)
Publication Details
- Journal
- Preparative Biochemistry & Biotechnology
- Published
- 2026-08-03
- DOI
- https://doi.org/10.1080/10826068.2026.2673213
- Primary Topic
- Acute Lymphoblastic Leukemia research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Tabriz University of Medical Sciences