CRISPR activation screen uncovers MARCKSL1 as a gauge for extracellular vesicle secretion
Extracellular vesicles (EVs) are crucial mediators of intercellular communication that originate from diverse membrane reservoirs. In a genome-wide CRISPR activation screen, Zhong et al. identified MARCKSL1—a membrane adaptor upregulated across many tumor types—as a potentiator of EV secretion from the plasma membrane, but not from mature endosomes.
Authors
- Roman I. Koning (ORCID: https://orcid.org/0000-0001-6736-7147)
- Anna E. George (ORCID: https://orcid.org/0000-0001-7009-1057)
- Cecilia de Heus (ORCID: https://orcid.org/0000-0001-8618-8451)
- Birol Cabukusta (ORCID: https://orcid.org/0000-0002-1187-3048)
- Peter A. van Veelen (ORCID: https://orcid.org/0000-0002-7898-9408)
- Ilana Berlin (ORCID: https://orcid.org/0000-0001-7917-1475)
- George M. C. Janssen (ORCID: https://orcid.org/0000-0001-9091-4030)
- Nalan Liv (ORCID: https://orcid.org/0000-0003-2654-9117)
- Rayman T.N. Tjokrodirijo (ORCID: https://orcid.org/0009-0007-4878-8385)
- Ruud H. Wijdeven (ORCID: https://orcid.org/0000-0002-3390-7384)
- Yingjie Zhong (ORCID: https://orcid.org/0000-0002-9296-5752)
- Manon Messchendorp
- Jacques Neefjes
- Jimmy J.L. Akkermans
- Frederik J. Verweij
Publication Details
- Journal
- Research Publications (Maastricht University)
- Published
- 2026-08-25
- DOI
- https://doi.org/10.1016/j.celrep.2026.117744
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00