Resveratrol-regulated genes through miRNAs in the gut epithelial cells may play an important role in the gut health and amelioration of EAE in mice 2255889

Abstract Introduction Resveratrol (RES) is an anti-inflammatory, anticancer, and antioxidant compound found abundantly in red grapes, blue berries, peanuts, etc. Methods In this study, we investigated the effect of RES on gene expression in the gut epithelial cells of mice with EAE. To this end, epithelial cells from small intestine (SI) and colons of mice with EAE treated with vehicle or RES were isolated. Transcriptome arrays using total RNAs from epithelial cells of SI and colons were performed and data obtained from the arrays were analyzed using Transcriptome Analysis Console software. Results Results showed that there were 1,591 (1165 in SI and 426 in colon) genes that were up- or down-regulated (>2-fold) in the epithelial cells of mice with EAE treated with RES, when compared to vehicles. We selected two genes, α-defensin and Mucin 3, for further analysis. Upon analysis of their expression in the gut epithelial cells, α-defensin were downregulated while Mucin 3 was upregulated. To understand the regulation of these two genes in the gut, we investigated the role of microRNAs (miRs). There were two miRs, miR-378 and miR-338, that showed strong binding affinity with α-defensin and Mucin 3 respectively. Upon analysis of their expression in epithelial cells, miR-378 was upregulated but miR-338 was downregulated, demonstrating their possible role in the regulation of α-defensin and Mucin 3 genes. Conclusion Data obtained from this study demonstrates a possible role of miRs in the regulation of genes in the gut epithelial that may play a role in improving the health of the gut and amelioration of EAE in mice. Funding Source P30GM154631, P20GM103641, R01ES030144 and R01AI160896 Topic Categories Immune Response Regulation: Molecular Mechanisms (IRM)