Implications of a dysfunctional meningeal lymphatic system in a mouse model of CSF1R-related leukoencephalopathy 2241999

Abstract Introduction Colony stimulating factor 1 receptor (CSF1R)-related leukoencephalopathy is an early onset neurodegenerative disease characterized by microglial deficits, abnormal glial responses, white matter pathology, and severe motor and cognitive decline. Methods At 8 months, mice lacking two copies of the Csf1r gene Fms-intronic regulatory element (FIRE) recapitulate some of the brain pathological features of CSF1R-related leukoencephalopathy, namely the deficits in microglia, astrogliosis, and demyelination. Of note, we detected deficits in lymphatic vasculature and altered macrophage activation in the meninges of Csf1rΔFIRE/ΔFIRE mice by flow, prior to the appearance of brain gliosis, neuronal dystrophy, and fine motor impairment. Results Eliminating the remainder CSF1R-expressing innate immune cells, including border-associated macrophages (BAMs), with PLX5622 at 2 months reshaped the immune crosstalk at the meningeal dura and restored lymphatic vascular expansion in Csf1rΔFIRE/ΔFIRE mice Conclusion Ongoing experiments in the lab will clarify the exact mechanisms through which dysfunctional innate immunity at the brain-meningeal interface and lack of meningeal lymphatic vasculature contribute to the development of brain pathology and impaired motor behavior in this model of CSF1R-related leukoencephalopathy. This work has unveiled novel molecular insights involving the meningeal lymphatic system that might be of therapeutic relevance to canonical central nervous system white matter diseases. Funding Source n/a Topic Categories Neuroimmunology (NEUR)