Single-cell blood immune cell atlas of immune diseases 2309369

Abstract Introduction Immune cells play multifaceted roles in the body, ranging from systemic and local pathogen surveillance to pathogenic auto-reactivity in conditions such as autoimmune diseases. While we can detect these immune abnormalities using transcriptomic methods, the complete cellular landscape of these diseases has not yet been comprehensively explored. Methods We profiled blood immune cells with single-cell RNA sequencing method. Results We generated single-cell profiles for 2,098,547 circulating immune cells from 231 individuals spanning 22 distinct immune-related conditions. We delineated five major immune lineages and characterized their transcriptomic alterations. We examined cross-lineage correlations in cellular responses and their associations with disease contexts. Conclusion This study present overall cellular architecture of blood immune cells, and provide broad landscape of systemic multicellular responses in immune-related diseases. Funding Source The Single Cell Atlas of Immune Diseases (SCAID) project Topic Categories Immune Mechanisms of Human Disease (HUM)