Making base editing accessible: Evaluating computational workflows for ABE-mediated gene knockout

Since the development of the first base editors, continued evolution has yielded a selection of editors that have been proved to be highly effective and precise in research and their first clinical use.1 Built on CRISPR technology, base editors utilize modified Cas proteins fused to a deaminase domain and directed by a single-guide RNA (sgRNA) to a specific genomic locus. Within a defined editing window, the deaminase catalyzes the conversion of one nucleotide to another without generating double-strand breaks (DSBs) (Figure 1).