Multimodal Raman and Fluorescence Lifetime Imaging: A New Analytical Approach for Resolving Timing-Dependent Metabolic Recovery in Endothelial Dysfunction

, complemented by nonspectral descriptors such as reduced cell volume and accelerated lipid droplet formation. Chemometric analysis utilizing principal component analysis, partial least-squares regression, and semiquantitative lipid profiling revealed a pronounced timing-dependent response to enalaprilat. Preventive treatment was found to preserve metabolic integrity, whereas post-treatment induced only partial restitution and generated a divergent metabolic trajectory. These findings demonstrate that early intervention leads to analytically distinguishable molecular states, underscoring the relevance of temporal profiling in endothelial diagnostics. By establishing a multimodal spectroscopic and chemometric framework, this work highlights the analytical power of integrative imaging for resolving subtle metabolic phenotypes and refining strategies for the objective evaluation of therapeutic efficacy.

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Publication Details

Journal
Analytical Chemistry
Published
2026-07-27
DOI
https://doi.org/10.1021/acs.analchem.6c01681
Primary Topic
Spectroscopy Techniques in Biomedical and Chemical Research
Type
article
Field-Weighted Citation Impact
0.00

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article

Multimodal Raman and Fluorescence Lifetime Imaging: A New Analytical Approach for Resolving Timing-Dependent Metabolic Recovery in Endothelial Dysfunction

Jürgen Popp, Aleksandra Borek-Dorosz, Małgorzata Barańśka, Marko Rodewald et al.
Analytical Chemistry
Spectroscopy Techniques in Biomedical and Chemical Research
article

Multimodal Raman and Fluorescence Lifetime Imaging: A New Analytical Approach for Resolving Timing-Dependent Metabolic Recovery in Endothelial Dysfunction

Jürgen Popp, Aleksandra Borek-Dorosz, Małgorzata Barańśka, Marko Rodewald, Julian Plitzko, Michael P. Schmitt, Hyeonsoo Bae, Anna Pieczara, Tobias Meyer‐Zedler, Krzysztof Brzozowski
article en

Abstract

, complemented by nonspectral descriptors such as reduced cell volume and accelerated lipid droplet formation. Chemometric analysis utilizing principal component analysis, partial least-squares regression, and semiquantitative lipid profiling revealed a pronounced timing-dependent response to enalaprilat. Preventive treatment was found to preserve metabolic integrity, whereas post-treatment induced only partial restitution and generated a divergent metabolic trajectory. These findings demonstrate that early intervention leads to analytically distinguishable molecular states, underscoring the relevance of temporal profiling in endothelial diagnostics. By establishing a multimodal spectroscopic and chemometric framework, this work highlights the analytical power of integrative imaging for resolving subtle metabolic phenotypes and refining strategies for the objective evaluation of therapeutic efficacy.

Analytical Chemistry
Jagiellonian University (PL), Helmholtz Institute Jena (DE), Leibniz Institute of Photonic Technology (DE), Friedrich Schiller University Jena (DE), Politecnico di Milano (IT)
Carl-Zeiss-Stiftung, Freistaat Thüringen, Deutscher Akademischer Austauschdienst, Deutsche Forschungsgemeinschaft, Fundacja na rzecz Nauki Polskiej, Narodowe Centrum Nauki, Bundesministerium für Forschung und Technologie, Narodowa Agencja Wymiany Akademickiej
Openalex Percentile: Top 10%
Spectroscopy Techniques in Biomedical and Chemical Research
DOIOpen Access PDFOpenAlex
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