Poly(3-hydroxybutyrate) Production from Wastewater: Microbial Pathways, Production Strategies, and Sustainability Perspectives

Abstract : The increasing accumulation of petroleum-based plastic waste and wastewater has intensified the need for sustainable waste management and biodegradable alternatives. Poly(3-hydroxybutyrate) (P3HB), a microbial biopolymer, has emerged as a promising substitute for conventional plastics. This article reviews the potential of wastewater as a renewable substrate for P3HB production, with emphasis on microbial pathways, production strategies, and sustainability aspects. It discusses different wastewater sources, P3HB-producing microorganisms, metabolic engineering approaches, production processes, and polymer recovery techniques, highlighting the factors that influence productivity and product quality. The integration of P3HB production into biorefinery systems and its contribution to resource recovery, greenhouse gas mitigation, and the circular bioeconomy are also addressed. Overall, wastewater-based P3HB production represents a sustainable and economically attractive approach for biodegradable polymer production, although further technological advances are required to support large-scale industrial implementation.