Seasonal Reef-Scale Variability in Seawater CO₂ Chemistry in the Presence and Absence of Seaweed Cultivation in Onna, Okinawa, Japan

Abstract Seaweed cultivation has been proposed as an active biogeochemical intervention to elevate seawater pH and create localized refugia from ocean acidification. To date, most studies have relied on autonomous sensors to contrast seawater pH inside and outside of seaweed cultivation plots. These studies provide important temporal observations but lack information about the spatial capacity for seaweed cultivation to provide pH refugia for surrounding habitats. This study investigated the spatiotemporal variability of seawater pH and CO₂ chemistry across the Onna-son reef, Okinawa, Japan, during the fall, winter, and spring seasons, with spring surveys coinciding with the peak extent of Mozuku ( Cladosiphon okamuranus ) cultivation. Relative to open-ocean conditions, seawater pH was elevated during 77–100% of afternoon observations in fall (up to + 0.10), 53% in winter (up to + 0.02), and 75–92% in spring (up to + 0.13). The greatest drawdown in dissolved inorganic carbon (DIC; − 44 µmol kg⁻ 1 ) and highest spatial pH variability (0.19) were observed during spring, the period of maximum seaweed cultivation, but coincided with reduced current velocities, small wave heights, and low mean sea level, conditions conducive to enhanced biogeochemical modification. Comparable pH elevations were observed during fall under similar temperature regimes, but higher current velocities, which suggest that seaweed cultivation may not be more effective than natural macroalgal habitats in elevating local seawater pH at this site. Future assessments of seaweed aquaculture as a mitigation strategy should explicitly incorporate local hydrodynamics and natural macroalgal influences, as these will impact the variability across both space and time.