Genome‐Wide Screening of Phase Separation Proteins and Functional Dissection of Candidate Proteins for Saline‐Alkali Stress Tolerance in Soybean
Summary statement We designed a bioinformatics pipeline combining PSPredictor and Metapredict to systematically identify phase separation proteins in soybean, yielding a total of 5515 candidates. Experimental validation confirmed that four nuclear‐localized proteins are capable of forming dynamic liquid‐like condensates through liquid–liquid phase separation both in vivo and in vitro. In addition, saline‐alkali tolerance assays revealed that GmBAF60b, GmFCA and GmPUX5 significantly enhance stress tolerance by lowering Na + /K + ratios and boosting antioxidant enzyme activities. These findings provide key genetic resources and mechanistic insights into LLPS‐mediated stress adaptation in crops.
Authors
- Xiaohuan Sun (ORCID: https://orcid.org/0000-0001-5655-131X)
- Haishan Liu (ORCID: https://orcid.org/0000-0001-5620-5898)
- Zhangxiong Liu (ORCID: https://orcid.org/0000-0002-3783-2754)
- Fujing Liu (ORCID: https://orcid.org/0009-0004-8422-354X)
- Xiaodong Ding (ORCID: https://orcid.org/0000-0003-3554-5855)
- Qiang Li (ORCID: https://orcid.org/0000-0002-4930-3075)
- Minglong Li (ORCID: https://orcid.org/0009-0004-1655-3088)
- Shuzhen Zhang (ORCID: https://orcid.org/0000-0002-3311-0092)
- Jialei Xiao (ORCID: https://orcid.org/0009-0009-1856-5257)
- Huang Xiaoya
- Xin Zhao
- Xin Liu
Institutions
- Northeast Agricultural University (CN)
- Shanghai Jiao Tong University (CN)
- Mudanjiang Medical University (CN)
- Heilongjiang Provincial Academy of Agricultural Sciences (CN)
- Mudanjiang Normal University (CN)
- Institute of Crop Sciences (CN)
Publication Details
- Journal
- Plant Cell & Environment
- Published
- 2026-07-26
- DOI
- https://doi.org/10.1111/pce.70778
- Primary Topic
- RNA Research and Splicing
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Heilongjiang Province