UAV Multispectral Estimation of Citrus Leaf Nitrogen Content by Integrating Object-Based Canopy Extraction and PSO-Optimized Machine Learning

Leaf nitrogen content (LNC) is an important physiological indicator for evaluating citrus nutritional status, photosynthetic capacity, and fertilization demand. However, conventional LNC determination mainly relies on field sampling and laboratory chemical analysis, which are destructive, time-consuming, labor-intensive, and limited in spatial continuity, making them unsuitable for large-scale real-time nitrogen monitoring in complex orchard environments. To achieve rapid and non-destructive estimation of citrus LNC, this study developed a UAV multispectral inversion framework integrating object-based canopy extraction and machine learning models. Field experiments were conducted in a citrus orchard in western Hubei Province, China. Multi-temporal UAV multispectral images were collected from April to October 2025, and ground measurements of citrus LNC were collected simultaneously. First, minimum distance classification (MDC), maximum likelihood classification (MLC), and object-based image analysis (OBIA) were used for land-cover classification of citrus orchard images, and their canopy extraction performance under complex orchard backgrounds was compared. Subsequently, multiple vegetation indices were calculated from the extracted citrus canopy spectra, and sensitive spectral features were selected through correlation analysis. Finally, seven models, including simple linear regression, quadratic regression, partial least squares regression (PLS), back propagation neural network (BP), extreme learning machine (ELM), particle swarm optimization-extreme learning machine (PSO-ELM), and particle swarm optimization-back propagation neural network (PSO-BP), were constructed to systematically evaluate the inversion performance of citrus LNC across the entire growth period. The results showed that: (1) OBIA achieved higher classification accuracy and temporal stability in citrus orchard land-cover classification, with overall accuracy ranging from 68.86% to 85.65% and Kappa coefficients ranging from 0.56 to 0.72, outperforming MDC and MLC. This indicates that OBIA can effectively reduce the interference of bare soil, grass, shadows, and other non-target objects on canopy spectral extraction. (2) The correlations between vegetation indices and LNC varied markedly among different growth stages, suggesting that the spectral response of citrus LNC has strong phenological dependence and that a single vegetation index is insufficient to stably characterize LNC variation across the whole growth period. (3) At the whole-growth-period scale, multi-index fusion models outperformed single-index models, among which EVI, TVI, and MTVI showed relatively strong cross-stage sensitivity. (4) Optimized machine learning models generally outperformed traditional regression models and unoptimized machine learning models. Among them, PSO-BP achieved the best performance, with a validation R2 of 0.68 and an RMSE of 1.54 g kg−1, representing an increase in R2 of 23.64% compared with the PLS model and 25.93% over the baseline BP model in terms of R2. Overall, this study demonstrates that OBIA-based canopy spectral quality improvement combined with PSO-optimized machine learning can effectively improve the stability and reliability of UAV multispectral estimation of citrus LNC under complex orchard backgrounds. The proposed framework provides technical support for citrus nitrogen diagnosis, precision fertilization, and intelligent orchard management.