Establishment of long-term feline oviduct epithelial spheroids in vitro culture system
This study aimed to characterize a long-term in vitro culture of feline oviduct epithelial spheroids (fOES) and to compare the effects of supplementation with fetal bovine serum (FBS) and bovine serum albumin (BSA) on fOES parameters. BSA improved structural preservation, reduced oxidative stress, and enhanced cell viability and motility. Despite morphological and metabolic changes, fOES preserved functional characteristics, supporting their use as a reliable in vitro model for reproductive studies in felids.
Authors
- Marie Saint‐Dizier (ORCID: https://orcid.org/0000-0002-4569-5665)
- Joanna Maria Gonçalves Souza‐Fabjan (ORCID: https://orcid.org/0000-0002-4872-1718)
- Magda Morais
- Ana Lucia R.S. Maia
- Agostinho S. Alcântara-Neto
- Daniella I. Chagas
Institutions
- Universidade Estadual do Ceará (BR)
- Université de Tours (FR)
- Centre National de la Recherche Scientifique (FR)
- Universidade Federal Fluminense (BR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Physiologie de la Reproduction et des Comportements (FR)
Publication Details
- Journal
- Reproductive Biology
- Published
- 2026-07-18
- DOI
- https://doi.org/10.1016/j.repbio.2026.101253
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro