Size-Tailored Nanospaces in Hierarchical Magnetic Core–Shell Microspheres for Lipase Immobilization and Phytosterol Ester Synthesis
(1.57-fold higher than the free enzyme) with 88.2% recovered activity. Crucially, in the sterically demanding esterification of phytosterols, the biocatalyst achieved 84.33% maximum conversion at 50 °C within 40 h. Furthermore, the rigid silica walls exerted a spatial "cage effect" that locked the enzyme active conformation, conferring exceptional thermal stability and reusability. This hierarchical support design offers a robust solution for macromolecular industrial biocatalysis.
Authors
- Baocai Xu (ORCID: https://orcid.org/0000-0002-0706-8863)
- Jin Mao (ORCID: https://orcid.org/0000-0002-4340-4836)
- Jie Ru Shi (ORCID: https://orcid.org/0000-0001-9521-3073)
- Zhonglin He
- Qianchun Deng (ORCID: https://orcid.org/0000-0002-5044-9047)
- Yuqi Fan (ORCID: https://orcid.org/0000-0002-9679-0929)
Institutions
- Hefei University of Technology (CN)
- Ministry of Education (SA)
- Ministry of Agriculture (CA)
- Chinese Academy of Agricultural Sciences (CN)
- Oil Crops Research Institute (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-07-16
- DOI
- https://doi.org/10.1021/acs.jafc.6c07410
- Primary Topic
- Enzyme Catalysis and Immobilization
- Type
- article
- Field-Weighted Citation Impact
- 0.00