Prognostic, not mediating: a six-cohort causal mediation analysis of immune signatures and tumour mutational burden under checkpoint blockade

This deposit contains the complete analytical pipeline, harmonised input data, and per-step result tables supporting the re-evaluation of combined-biomarker assumptions in the tumour mutational burden (TMB)-survival association under PD-(L)1 blockade. The analysis re-uses six published, de-identified checkpoint-blockade cohorts in urothelial carcinoma, renal cell carcinoma, melanoma, and non-small cell lung cancer (NSCLC), with 622 samples entering at least one analytical layer, together with one single-cell deconvolution reference (Chen 2020) and one single-cell audit dataset (Sade-Feldman 2018).