Population Genetic Data for 23 STR Loci of the Maya Chortí Ethnic Group in Honduras

Background: The Maya Chortí are a Mesoamerican Indigenous group of approximately 33,256 individuals in Copán and Ocotepeque, western Honduras, historically, linguistically, and culturally linked to the Classic Maya tradition of Copán. No population-specific autosomal short tandem repeat (STR) reference dataset had previously been available, requiring forensic calculations to use non-representative databases. Methods: Allele frequencies for 23 autosomal STR loci were estimated in 100 unrelated Maya Chortí individuals from Copán and Ocotepeque. DNA from blood on FTA cards was amplified with the PowerPlex Fusion 6C System. Hardy–Weinberg equilibrium (HWE), pairwise linkage disequilibrium (LD), diversity indices, forensic parameters, inter-population FST, and random match probabilities with and without NRC-II θ-correction (θ = 0.01 and 0.03) were calculated using Genepop, Arlequin, and STRAF. Results: A total of 212 alleles were detected. Expected heterozygosity was high across the panel. After Bonferroni correction, no locus departed from HWE, and no locus pair showed significant LD. The combined random match probability was 1.17 × 10−23, very low, and remained highly discriminating under NRC-II θ-correction; the combined chance of exclusion exceeded 99.99%. Conclusions: This study provides the first autosomal STR reference database for the Maya Chortí of Honduras, enabling population-specific likelihood ratio estimation in forensic identification, paternity testing, and kinship analysis, while expanding the genetic characterization of Mesoamerican Indigenous populations under CODIS/ESS standards.