Gating effects of a Cav2.3 calcium channel variant linked to developmental and epileptic encephalopathy

Pathogenic Cav2.3 variant L228P exhibits complex alterations in channel gating • The Cav2.3(L228P) mutant shows a hyperpolarizing shift in half-activation voltage • The L228P substitution causes a hyperpolarizing shift in half-inactivation potential • The mutant channel exhibits an increase in window current • Inactivation kinetics or recovery from inactivation are not altered in Cav2.3(L228P)Khousakoun et al., 2 Khousakoun et al., 3