Self-Assembed G-Quadruplex Nanowires for Energy Transfer over Micrometers

G-quadruplex-based nanowires (G-wires) are promising candidates for high-performance photonic and electronic nanomaterials, yet their utility is restricted by the discontinuous phosphodiester linkages inherent in the current assembly methods. These discontinuities impair structural stability, limit axial growth, and compromise the long-range energy transfer efficiency. Herein, by employing hairpin-structured splint strands to template the high-yield cyclization of poly(dC) DNA, we enable rolling circle amplification to produce ultralong poly(dG) DNA that spontaneously self-assembles into micrometer-scale G-wires with a continuous phosphate backbone. Furthermore, a nucleic acid-specific fluorescent probe, thiazole orange (TO), showed a highly efficient long-range energy transfer through G-wires, compared with double-stranded DNA (dsDNA) and G-quadruplex monomers.