Fully Complementary Interactions Between LmiRNA and mRNA of Human Genes

Londin et al. discovered a novel group of miRNAs, referred to as LmiRNAs, whose properties had been studied little for unknown reasons. In this study, we examine fully complementary interactions between LmiRNAs and mRNAs of human genes. Using the MirTarget program, we identified a significant number of target genes showing unique interaction with LmiRNAs. Among the 3707 LmiRNAs, fully complementary binding sites (BSs) were found in the 5′UTR of 75 target genes, with their interactions exhibiting high free energy. Fully complementary LmiRNA binding sites were located within the CDS of 81 target genes, while only seven LmiRNAs were found to bind to the 3′UTR of target genes. The KIFC3, PHF15, RPL15, and SNX11 genes were found to encode both LmiRNA-5p and LmiRNA-3p, which actively bind to their respective mRNAs. While the mRNA of most genes was targeted by only a single LmiRNA, the BMP8B, FGFRL1, and SDC3 genes included mRNAs bound by the specific pair ID00121.5p and ID02992.5p. These results expand our understanding of LmiRNAs and support their potential as diagnostic and therapeutic agents for various diseases.