Evaluating Ecological Integrity in Andean High-Mountain Streams Using a Multiplicative Water Quality Index: Roles of Seasonal Hydrology and Benthic Assemblages

High-mountain lotic ecosystems in the tropical Andes are increasingly threatened by agricultural expansion and seasonal hydrological variability. This study evaluates structural water quality shifts and benthic macroinvertebrate community assembly across an environmental degradation gradient in the La Chorrera micro-basin (Boyacá, Colombia). A weighted multiplicative Water Quality Index (mWQI), originally developed in previous literature, was implemented and validated alongside biotic scores (BMWP/Col) and Canonical Correspondence Analysis (CCA) across 16 sampling points during contrasting wet and dry seasons. The results demonstrate that intense precipitation during the wet season introduces a volumetric dilution effect, maintaining high ecological quality scores (mWQI > 80.0) even in deeply modified agricultural sectors. Conversely, severe baseflow contraction during the dry season triggers pollutant concentration, driving total phosphorus to hyper-eutrophic peaks (9.20 mg/L) and forcing a complete mathematical collapse of the index (mWQI = 0.00). The CCA biplot confirmed that seasonal chemical filters combined with hypoxia (<60% oxygen saturation) eliminate highly responsive macroinvertebrate orders (Ephemeroptera, Plecoptera, Trichoptera), leading to absolute dominance of hypoxia-tolerant opportunists (Chironomidae and Oligochaeta). The benthic community shifted from physical stress (sedimentation) during the wet season to chemical stress (nutrient toxicity and oxygen depletion) during the dry season. The multiplicative framework effectively isolates localized ecological tipping points, providing a high-sensitivity baseline tool for targeted watershed restoration in tropical alpine regions.