Protocol for somatic cell nuclear transfer in the common marmoset

Somatic cell nuclear transfer (SCNT) in non-human primates is highly inefficient, as many reconstructed embryos arrest during preimplantation development. Here, we provide a protocol for performing SCNT in the common marmoset. We describe steps for oocyte collection, in vitro maturation, enucleation, and nuclear transfer using fibroblast or cumulus donors. We then detail procedures for oocyte activation, triple epigenetic enhancement, and embryo culture up to the blastocyst stage. For complete details on the use and execution of this protocol, please refer to Matoba et al. 1