Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling

Oxidative stress and inflammation, in particular, are crucial factors in the pathogenesis of myocardial infarction (MI). Isoproterenol (ISO) is frequently preferred for creating experimental MI models because it reflects the pathophysiology of MI in humans. The purpose of this study was to identify potential alterations in OTULIN and progranulin (PRGN) levels at various stages during and following cardiac remodeling in experimental MI caused by ISO. The study consisted of control groups and ISO groups (ISO-6-h, ISO-24-h, ISO-3-day, and ISO-7-day) representing important time points in experimental MI and subsequent cardiac remodeling. The experimental MI model induced by ISO was validated by increased serum cardiac markers and histopathological changes in the heart tissue. Furthermore, it was determined that oxidative stress and inflammation, important factors in MI pathophysiology, emerged in the heart tissue after ISO administration. However, in the ISO-7-day group, a significant reduction in oxidative stress and inflammation was observed along with cardiac remodeling. In the experimental MI model, a time-dependent decrease in cardiac OTULIN levels was observed, while conversely, an increase in cardiac PRGN levels was detected. These time-dependent dynamic changes in cardiac OTULIN and PRGN suggest that both may play a role in processes related to MI and cardiac remodeling. In conclusion, endogenous OTULIN and PRGN may be associated with oxidative stress and inflammation in cardiac remodeling during and after experimental MI. This reveals the potential of OTULIN and PRGN as cardiac biomarkers in MI prognosis and suggests they could also be molecular targets for therapeutic strategies.