Investigation of the Effect of the Tri-Set Training Method on Plasma mir-1, mir-128a, mir-133, and mir-206 Levels

The miRNAs (Micro Ribonucleic Acid) mir-1, mir-128a, mir-133, and mir-206 are specifically associated with muscle hypertrophy and play key roles in its development. The aim of this study is to investigate the effects of the tri-set training method on the expression levels of mir-1, mir-128a, mir-133, and mir-206. Twenty-six volunteer athletes participated in the study. Anthropometric measurements were performed on the first day. Blood samples were collected before training and a 12-repetition maximum test (12RM) protocol was applied. On the second day, a maximum of 12 repetitions of Full Squat (SQ), Deadlift (DL), and 45° Leg Press (LP) movements were tested. On the third day, the training protocol was applied. On the fourth day, post-training blood samples were obtained. Expression levels of mir-1, mir-128a, and mir-206 increased after training, but these changes were not statistically significant (P<0.05). Correlation analysis of pre- and post-training expression levels shown significant positive correlations between mir-1 (r= 0.760) and mir-128a (r= 0.737). Tri-set training elevated the levels of mir-1, mir-128a and mir-206. These results suggest a potential relationship between muscle hypertrophy and these miRNAs. Further investigations with larger sampling sizes are required to confirm these findings.