Human neonatal MR1T cells have more diverse TCR repertoires but reduced bacterial recognition than adult MR1T cells
Bacterial sepsis is a leading cause of neonatal mortality. Pro-inflammatory MR1-restricted T (MR1T) cells may help protect from sepsis by recognizing bacterial pathogens producing the canonical MR1 antigen 5-OP-RU. Most adult MR1T cells are mucosal-associated invariant T (MAIT) cells expressing a semi-invariant TCRα, while neonatal MR1T cells express diverse TCRα chains. Here, we perform combined single-cell RNA-sequencing and TCR repertoire analyses on MR1/5-OP-RU tetramer-positive cells from neonatal cord blood (CB) and adult blood. Compared to adult MR1T cells, CB MR1T cells exhibit greater TCR diversity, reduced cytotoxic and proinflammatory gene expression, diminished bacterial recognition and reduced binding to MR1/5-OP-RU. Structural analysis of a CB MAIT TCR reveals decreased β chain contribution to the TCR-MR1 interface relative to an adult MAIT TCR. These findings demonstrate developmental stage-specific differences in MR1T cell repertoire, function and MAIT TCR structure with implications for neonatal sepsis.
Authors
- David Lewinsohn (ORCID: https://orcid.org/0000-0001-9906-9494)
- Wael Awad (ORCID: https://orcid.org/0000-0002-7597-2133)
- Gwendolyn Swarbrick (ORCID: https://orcid.org/0000-0001-5786-1256)
- Deborah A. Lewinsohn (ORCID: https://orcid.org/0000-0003-1349-3775)
- GW McElfresh
- Megan Null (ORCID: https://orcid.org/0009-0001-9584-4606)
- Dylan Kain (ORCID: https://orcid.org/0000-0001-5890-2742)
- Benjamin N. Bimber (ORCID: https://orcid.org/0000-0001-8092-3080)
- Katherine H. Rott (ORCID: https://orcid.org/0000-0001-5349-2473)
- Jamie Rossjohn (ORCID: https://orcid.org/0000-0002-2020-7522)
- Kean Chan Yew Poa (ORCID: https://orcid.org/0000-0003-4090-9561)
- Conor McNeice
- Gregory Boggy
- Meghan Cansler
Institutions
- Oregon National Primate Research Center (US)
- Oregon Health & Science University (US)
- University of Toronto (CA)
- Australian Regenerative Medicine Institute (AU)
- Children's Cancer Therapy Development Institute (US)
- Vaccine and Gene Therapy Institute
- Monash University (AU)
- Cardiff University (GB)
Publication Details
- Journal
- Nature Communications
- Published
- 2026-06-29
- DOI
- https://doi.org/10.1038/s41467-026-74998-7
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Veterans Affairs
- National Institutes of Health
- Canadian Institutes of Health Research
- National Institute of Allergy and Infectious Diseases