Characterization of genomic diversity in bacteriophages infecting Rhodococcus
Bacteriophages are ubiquitous and highly genetically diverse biological entities. Here we describe the isolation and bioinformatic characterization of 56 phages isolated on two Rhodococcus spp. They include both lytic and temperate phages and are grouped with previously described Rhodococcus phages into six clusters and 16 singletons based on genome similarity. Their genome sizes range from 43.9 kbp to 142 kbp and they have a G + C content ranging from 41.2% to 68.4%. Some of the Rhodococcus phages are more closely related to phages isolated on non-Rhodococcus Actinobacteria hosts than they are to phages isolated from the same host genus, demonstrating complex evolutionary histories. This study further expands the growing field of Actinobacteriophage genomics.
Authors
- Brianna Empson
- Amanda C. Freise (ORCID: https://orcid.org/0000-0002-8704-5185)
- Daniel A. Russell (ORCID: https://orcid.org/0000-0001-6894-2748)
- Deborah Jacobs‐Sera (ORCID: https://orcid.org/0000-0001-7838-3257)
- Ana E. Garcia‐Vedrenne (ORCID: https://orcid.org/0000-0002-3518-1564)
- Rebecca A. Garlena (ORCID: https://orcid.org/0000-0003-3245-2629)
- Randall J. DeJong (ORCID: https://orcid.org/0000-0001-5660-9227)
- Maisam Ghannam
- James A. Bruns
- Graham F. Hatfull (ORCID: https://orcid.org/0000-0002-6705-6821)
- Dominic R. Garza
- Ann M. Findley
- Steven G. Cresawn (ORCID: https://orcid.org/0000-0002-2162-562X)
- Karen K. Klyczek (ORCID: https://orcid.org/0000-0001-7882-0429)
- Colin M. Lewis
- Isabel Light
- Daria L. Di Blasi (ORCID: https://orcid.org/0009-0002-3640-2178)
- Jorge Alfred Bonilla (ORCID: https://orcid.org/0000-0003-0713-9454)
- Sally Molloy
Institutions
- James Madison University (US)
- University of Wisconsin–River Falls (US)
- University of California, Los Angeles (US)
- Calvin University (US)
- University of Pittsburgh (US)
- Michigan United (US)
- University of Louisiana at Monroe (US)
- University of Maine (US)
Publication Details
- Journal
- PLoS ONE
- Published
- 2026-06-29
- DOI
- https://doi.org/10.1371/journal.pone.0352686
- Primary Topic
- Bacteriophages and microbial interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Howard Hughes Medical Institute
- James Madison University
- National Institutes of Health