Detection of enteric protozoa using multiplex polymerase chain reaction technique in neonatal Hanwoo calves with diarrhea

accounting for approximately 68.7% of total mortality (Kim et al., 2015).Among these, protozoan infections are recognized as significant etiological agents of en-teric disease worldwide.In Korean native calves, the prevalence of diarrhea caused by protozoan parasites is high at 53.1%, with Cryptosporidium spp., Giardia spp., and Eimeria spp.identified as the principal infectious agents.Specifically, Eimeria spp.have been detected in 59.6% of diarrheic calves and are frequently observed in mixed infections, occurring in approximately 19.5% of cases (Lee et al., 2019).Eimeria spp.are widespread parasite infections in cattle, and oocysts present in the environment can cause infection in calves (Das et al., 2015).Infection in calves with a high number of oocysts can cause clinical KJVS