Protocol for an AlCl3-induced Alzheimer’s model in zebrafish larvae with optimized pH and behavioral assessment
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that impairs cognition and memory. Current protocols for establishing AD models in zebrafish ( Danio rerio ) larvae are limited and lack comprehensive detail. We present a refined zebrafish model, optimizing pH control of AlCl 3 solutions and improving the behavioral assays to ensure a consistent AD-like phenotype. We describe steps for breeding embryos, testing behavior, selecting and treating larvae, and analyzing data. This protocol facilitates high-throughput screening of potential therapeutic drugs and genes for AD.
Authors
- Liangbiao Chen (ORCID: https://orcid.org/0000-0002-0717-8536)
- Jie Zhang (ORCID: https://orcid.org/0009-0006-3569-1587)
- Lufang Zou
- Wei Li (ORCID: https://orcid.org/0000-0002-2493-1083)
- Ruiqin Hu (ORCID: https://orcid.org/0000-0002-7738-6074)
- Zequan Lin
- Lijia Pan
Institutions
- Shanghai Ocean University (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2026-06-26
- DOI
- https://doi.org/10.1016/j.xpro.2026.104657
- Primary Topic
- Zebrafish Biomedical Research Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00