AI-Accelerated Structure Elucidation of Boavistamides A <b>–</b> C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde
Boavistamide A ( 1 ), a new alkyne-containing cyclic depsipeptide featuring the rare 3-amino-2-methyl-7-octynoic acid (AMOYA) moiety, was discovered along with two structurally related analogs, boavistamides B and C ( 2 and 3 ), from a filamentous marine cyanobacterium collected on Boa Vista Island, Cabo Verde. Their isolation was guided by antiplasmodial activity, GNPS MS/MS molecular networking, LC-MS profiling, and dereplication using the MarinLit database. The planar structures of boavistamides A–C ( 1 – 3 ) were elucidated through comprehensive HRMS and 1D/2D NMR analyses, with annotation support from AI-based tools SMART-NMR 2.1 and DeepSAT. The absolute configurations were established using Marfey’s analysis and l -Phe-OMe coupling, complemented by NMR-based conformational studies. Boavistamides A and B exhibited moderate antiplasmodial activity with no mammalian cell cytotoxicity. Microscopic observations and metagenomic binning identified the producer strain as belonging to the genus Okeania (Microcoleaceae). These results expand the chemical diversity of AMOYA-containing cyanobacterial metabolites and highlight the utility of integrated metabolomics and AI-assisted workflows for natural product discovery from environmental samples.
Authors
- Byeol Ryu (ORCID: https://orcid.org/0000-0002-3405-2875)
- Krittikorn Kümpornsin (ORCID: https://orcid.org/0000-0001-9427-7139)
- Thaiz R. Teixeira
- Case W. McNamara (ORCID: https://orcid.org/0000-0002-5754-3407)
- Evgenia Glukhov (ORCID: https://orcid.org/0000-0001-6513-9909)
- Marine Cuau (ORCID: https://orcid.org/0000-0003-1728-7901)
- Pedro N. Leão (ORCID: https://orcid.org/0000-0001-5064-9164)
- Mariana Laureano de Souza (ORCID: https://orcid.org/0000-0003-1886-3878)
- Nicole E. Avalon (ORCID: https://orcid.org/0000-0003-3588-892X)
- Jehad Almaliti (ORCID: https://orcid.org/0000-0002-3562-0846)
- William H. Gerwick (ORCID: https://orcid.org/0000-0003-1403-4458)
- Elizabeth A. Winzeler (ORCID: https://orcid.org/0000-0002-4049-2113)
- Adriana Rego (ORCID: https://orcid.org/0000-0001-9647-1197)
- Joseph Chen (ORCID: https://orcid.org/0009-0008-9481-2099)
- Conor R. Caffrey (ORCID: https://orcid.org/0000-0002-3234-9608)
- Vitor Vasconcelos
- Alma Trinidad-Javier
- Mai Shingyoji
Institutions
- Scripps Institution of Oceanography (US)
- Universidade do Porto (PT)
- University of California, Irvine (US)
- Mahidol University (TH)
- University of California San Diego (US)
- Scripps (United States) (US)
- California Institute for Biomedical Research (US)
- Center for Discovery (US)
- University of Montana (US)
Publication Details
- Journal
- Journal of Natural Products
- Published
- 2026-06-24
- DOI
- https://doi.org/10.1021/acs.jnatprod.6c00391
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00