A chromosomal-level genome assembly of Batocera lineolata Chevrolat, 1852 (Coleoptera: Cerambycidae)

The white-striped longhorned beetle, Batocera lineolata, is an important forestry pest, with larvae that damage vascular and woody tissues, frequently leading to tree mortality. The lack of a high-quality genome has constrained research into the mechanisms underlying its damage. Here, we report a chromosome-scale genome of B. lineolata, constructed through an integrated approach combining PacBio HiFi, Hi-C, and Illumina RNA sequencing data. The assembled genome of B. lineolata spans 327.96 Mb across 18 scaffolds, with a scaffold N50 of 34.45 Mb. Notably, 94.5% of the assembly (309.92 Mb) was anchored to 10 chromosomes. Synteny analysis identified chromosome 10 as the X chromosome and confirmed its conservation across Coleoptera. BUSCO analysis indicated a high genome completeness of 98.8%, comprising 98.7% single-copy and 0.1% duplicated genes. Repetitive elements accounted for 28.04% (91.97 Mb) of the genome, and 12,030 genes were annotated. This genome provides a foundation for studying the ecology and genetics of B. lineolata and for developing more effective wood-boring pest control strategies.