Transparent film and electrospun PVA-HA nanofibers incorporated MSNs loaded TET/ERY for wound healing: characterization and in vitro study

In the recent years, the use of mesoporous silica nanoparticles (MSNs) in drug delivery has a great attention, due to several unique properties including biocompatibility, high drug loading capacity, etc. In this study, tetracycline hydrochloride (TCH) and erythromycin (ERY) loaded MSNs were prepared and then nanofibers and transparent film were fabricated as a drug delivery system. Several characterizations including dynamic light scattering (DLS), scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR) and mechanical strength was applied for free MSNs and final nanocomposites. Furthermore, in vitro drug release and cytotoxicity effects were investigated. Also, the antibacterial properties of nanocomposite were investigated against gram-positive and gram-negative bacteria. Based on our results, incorporation of MSNs into nanofiber and transparent film leads to increase the mechanical strength. The drug release profile indicated that both free tetracycline (TET) and ERY released entirely in 8 h, while antibiotic loaded in MSNs nanofibers and transparent film showed a control release manner. According to MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay on any of prepared nanocomposite showed no cytotoxicity effects against HUVEC cells. Nanocomposite containing TET demonstrated a good antibacterial activity against Staphylococcus aureus and Escherichia coli, while nanocomposite containing ERY effective only against S. aureus. By overcoming the limitations of conventional rapid-release antibiotics, this study provides a critically needed solution for prolonged, localized drug delivery, enhancing treatment efficacy for wound care.