Fire Heat and Ash Deposition Regulate Post-Fire Soil Bacterial Community Recovery and Predicted Function Potential

Disentangling the combined effects of heat and ash in natural forest fires is challenging, hindering understanding of soil microbial post-fire responses. A 90-day simulated fire experiment with 16S rRNA sequencing monitored bacterial communities and functional potential in topsoil (0–10 cm) and subsoil (10–20 cm) under seven treatments: blank control/BC, dry ash/DA, wet ash/WA, low-intensity heating/LH, high-intensity heating/HH, charcoal smoldering combustion/CSC, and Fire, with samples collected every ten days. Results: (1) α diversity declined mainly in the topsoil, with reductions of 12.04–19.82% for Shannon, 1.23–2.86% for Simpson, and 16.03–31.34% for the Chao index. Subsoil only declined under CSC. (2) Both heating and ash treatments increased the relative abundance of low-abundance and endemic taxa. Heating significantly enriched thermotolerant, xerotolerant, and oligotrophic taxa, such as Ramlibacter. (3) Topsoil heating treatments separated from BC (p ≤ 0.01), ash clustered with BC; pH and water content drove differentiation (p ≤ 0.05). (4) Topsoil predicted function potential showed early suppression (0–20 d), mid recovery (30–60 d), and late enhancement (70–90 d) for most treatments, except WA with sustained suppression. Heat determines disturbance depth and initial bacterial loss, while ash reshapes soil properties to influence community reassembly, acting as sequential but distinct environmental filters, providing a framework for post-fire bacterial community reorganization.