High-Quality Genome Assembly of the White King Pigeon for Genetic Reference of Chinese Market Pigeon Breeds

Abstract This study was conducted to generate a high-quality chromosome-level genome assembly of a female White King pigeon using a combination of Illumina short pair-end reads; HiFi reads and Hi-C technologies. Using the reference genome,Over 22,685,232 single nucleotide polymorphisms (SNPs) and 2,466,122 insertion-deletions (InDels) were identified. The genome sequence with a total length of 1.09 Gb was located on 29 chromosomes, accounting for 98.59%, while the number of corresponding sequences was 1,270, accounting for 61.53%. Among the sequences located on chromosomes, the length of the sequences that can determine the order and direction was 1.06 Gb, accounting for 96.8% of the total length of the sequences located on chromosomes. Sixty-four pigeons from six breeds of Chinese mainstream market were investigated and approximately 94.67% high-quality sequences(Q30 > 90%), with a mapping rate over 99.12% for each individual were observed, with a genome coverage of 99.12%. further contributing to the public database. This dataset provides valuable resources for studying genetic diversity and adaptation for the cultivation of new pigeon breeds.