Implementation of activity assays in microfluidics at high temperature for esterase/lipase screening
The aim of this work is to describe the development and optimization of a microfluidic-based screening procedure for the detection of metagenomic esterases and lipases from thermal environments. The proposed approach combines droplet microfluidics with fluorogenic substrates to enable high-throughput and sensitive identification of enzymatic activity at elevated temperatures. Key parameters affecting droplet generation, assay stability, and signal detection were systematically evaluated and optimized. This procedure provides a robust platform for the functional screening of thermophilic metagenomic libraries and represents a valuable tool for the discovery of novel biocatalysts with potential biotechnological applications.
Authors
- Aurélio Hidalgo (ORCID: https://orcid.org/0000-0001-5740-5584)
- María L. Rúa (ORCID: https://orcid.org/0000-0001-5952-2442)
- Olalla López-López
- Liisa van Vliet (ORCID: https://orcid.org/0000-0002-5196-7073)
- Florian Hollfelder (ORCID: https://orcid.org/0000-0002-1367-6312)
- José Berenguer (ORCID: https://orcid.org/0000-0002-9689-6272)
- Ana Torrado (ORCID: https://orcid.org/0000-0002-5325-0738)
- Jacobo Cruces
Institutions
- University of Cambridge (GB)
- Global Alliance for Livestock Veterinary Medicines (GB)
- Direction de la Recherche Technologique (FR)
- Centro de Biología Molecular Severo Ochoa (ES)
- Universidade de Vigo (ES)
- Universidad Autónoma de Madrid (ES)
Publication Details
- Journal
- Biocatalysis and Biotransformation
- Published
- 2026-06-18
- DOI
- https://doi.org/10.1080/10242422.2026.2686654
- Primary Topic
- Innovative Microfluidic and Catalytic Techniques Innovation
- Type
- article
- Field-Weighted Citation Impact
- 0.00