Role of hand disinfection of dentists in interrupting cross-transmission of infections in dental practice

Abstract Introduction : It is mandatory to perform meticulous hygienic disinfection of dentists’ hands. Dental personnel unintentionally transfer microorganisms from the patient’s mouth to adjacent surfaces, and vice versa. Aim : The aim of the present study was to determine the presence of resident and transient skin microbiota on the hands of dentists after performing hygienic hand disinfection. Materials and methods : A prospective epidemiological and microbiological study was conducted among 60 dentists in Plovdiv from May to June 2025. A short survey was also administered to assess basic knowledge of hygienic hand disinfection. After hand disinfection with alcohol-containing antiseptic, samples were collected with a dry sterile swab before donning gloves and manipulating the specimen. Results : Coagulase-negative staphylococci in microbial counts below 10 5 colony-forming units (CFU) were detected in 26% (n=16) and above 10 5 CFU in 20% (n=12). Gram-negative enteric bacteria were cultured in microbial counts below 10 5 CFU in 13.3% (n=8) and at 10 5 CFU in 1.7% (n=1). Molds were also grown in 3.3% (n=2). Conclusion : The presence of coagulase-negative staphylococci and Gram-negative bacteria in high microbial counts raises concerns about the disinfection quality. Regular and proper disinfection significantly reduces the risk of contamination and protects both the patient and the dental staff.