Protein RNA Binding

<p><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Arial",sans-serif"><b>ASBMB Learning Objectives</b> (<a href="https://www.asbmb.org/education/core-concept-teaching-strategies/foundational-concepts/structure-function"><span style="color:#1155cc">https://www.asbmb.org/education/core-concept-teaching-strategies/foundational-concepts/structure-function</span></a>) </span></span></span></p> <p><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Arial",sans-serif">2. Structure is determined by several factors</span></span></span></p> <ul> <li style="margin-left:8px"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Arial",sans-serif">Students should be able to <b>recognize the repeating units</b> in biological macromolecules and be able to discuss the <b>structural impacts of the covalent and noncovalent interactions involved</b> <i><span lang="EN" style="font-size:12.0pt">(Introductory)</span></i><span lang="EN" style="font-size:12.0pt">.</span></span></span></span></li> <li style="margin-left:8px"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Arial",sans-serif">Students should be able to <b>use various bioinformatics approaches to analyze</b> macromolecular primary <b>sequence and structure</b> <i><span lang="EN" style="font-size:12.0pt">(Intermediate)</span></i><span lang="EN" style="font-size:12.0pt">.</span></span></span></span></li> </ul> <p style="margin-top:16px; margin-bottom:16px"><span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif">3. Structure and function are related</span></span></span></p> <ul style="margin-top:16px; margin-bottom:16px"> <li style="margin-top:16px; margin-bottom:16px; margin-left:8px"><span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif">Students should be able to use <b>mechanistic reasoning to explain how an enzyme </b>or ribozyme <b>catalyzes a particular reaction</b> <i><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%">(Introductory)</span></span></i><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%">.</span></span></span></span></span></li> </ul> <p><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Arial",sans-serif">4. Macromolecular interactions</span></span></span></p> <ul style="margin-top:16px; margin-bottom:16px"> <li style="margin-top:16px; margin-left:8px"><span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif">Students should be able to discuss the <b>interactions between a variety of biological molecules</b> (including proteins, nucleic acids, lipids, carbohydrates and small organics, etc.) and <b>describe how these interactions impact specificity or affinity</b> leading to changes in biological function <i><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%">(Intermediate)</span></span></i><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%">.</span></span></span></span></span></li> <li style="margin-bottom:16px; margin-left:8px"><span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Arial",sans-serif">Students should be able to <b>predict the effects of either mutation or ligand structural change on the affinity of binding </b>and design appropriate experiments to test their predictions <i><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%">(Upper)</span></span></i><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%">.</span></span></span></span></span></li> </ul>