Quantification of Stress- and Resistance-Related Metabolites in Barley Leaves ( <i>Hordeum vulgare</i> L.) Infected with <i>Bipolaris sorokiniana</i> via UHPLC-MS/MS _MRM

High Resolution Image Download MS PowerPoint Slide This study investigated the quantitative changes in 33 stress- or resistance-related metabolites induced by Bipolaris sorokiniana in barley leaves of quantitatively resistant and susceptible barley lines of the multiparental nested association mapping (NAM) population HEB-25. The analyses were based on ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Twenty-nine infected and noninfected barley genotypes were analyzed at four different time points after inoculation. The method provided quantification of hordatines, phenolamides, hydroxycinnamic acids, flavone glucosides, hydroxynitrile glucosides, apocarotenoids, and indole derivatives. In leaves infected with B. sorokiniana, phenolamide levels were elevated compared to noninfected plants. A correlation between metabolite levels and the severity of infection showed that the more resistant barley lines contained higher amounts of hordatines and hordatine glucosides.